10X Phosphate Buffered Saline

• 0.58M Na 2HPO 4 (82.3g)
• 0.17M NaH 2PO 4 (23.5g)
• 0.08M NaCl (4.7g)
• Add distilled water till 1L
• Yields 750mM PBS

Dilute 1:10 to yield 1X Phosphate Buffered Saline which is 75mM