Blocking Buffer and Washing Buffer (formerly Assay Buffer)

Blocking Buffer

0.2% Aurora TM Blocking Reagent

1X Phosphate Buffered Saline

0.1% Tween-20

10X Washing Buffer(formerly Assay Buffer)

200mM TRIS (pH 9.8)

10mM MgCl 2

15.76g TRIS-HCl

1.015g MgCl 2

Bring to 500ml and adjust pH to 9.8 with 1M NaOH

Dilute 1:10 to yield 1X Washing Buffer